4. Install the free end of the adapter tubing onto the calibration adapter.

5. Press the calibration adapter onto the BioStage inlet until a fi rm seal is established.

6. Connect the BioStage outlet to one end of the ?-inch ID tubing.

7. Insert the small end of a second Luer adapter into the free end of the ?-inch

tubing.

8. Insert the large end of the second Luer adapter into one end of the 3/8-inch

sampling tubing and the pump inlet adapter into the other end.

9. Insert the pump inlet adapter into the pump inlet.

10. Calibrate the sample pump to 28.3 L/min for a Standard BioStage or

14.15 L/min for BioStage 200 (see calibration procedure in QuickTake 30 operating

instructions 40079).

11. When calibration is complete, remove the calibration adapter and tubing and

fl owmeter.

The calibration adapter and rotameter are for use with both models of BioStage. See Accessories/

Replacement Parts.

Sample

Read the following before sampling.

? Sanitize hands or wear disposable gloves. Sanitize hands and clean the BioStage

Impactor in between samples.

? Never use agar that has expired, displays visible cracks, or has been contaminated.

? Allow agar plates to warm up to ambient temperature (approximately 20 minutes)

before use.

? When sampling indoors, close all doors and windows that could affect airfl ow in the

sampling area.

1. The BioStage outlet should be connected to

the pump inlet with fl exible tubing or using

the Mounting Bracket accessory (Cat. No.

228-9531); see Operating Instructions 40066

supplied with that accessory (see right).

2. Remove the inlet cone and jet classifi cation

stage from the BioStage. Place a new,

unexposed agar plate into the base plate (see

Assemble with Media). Remove the lid from

the agar plate and place in a clean resealable

bag. Reassemble the impactor.

3. Turn on the pump and sample for 2 to 5 minutes.

Sampling for too long can cause overgrowth of sample. Sampling times that are

too short can cause false negatives.